Measure another 20 cm3 pureed potato into it. Wear eye protection and protect clothing from hydrogen peroxide. Investigating an enzyme-controlled reaction: This heat energy is transferred to the environment. Individual students may then have time to gather repeat data.
Now that I have carried out the repeats and plotted the points on the rate of reaction graph, I can see that the graph is in fact clearly linear. It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.
Individual enzymes are named by adding ASE to the name of the substrate with which they react. When the products have been released, the enzyme is ready to bind with a new substrate.
So therefore I will be using a burette instead of a measuring cylinder, mainly because the scale division is larger. More collisions are therefore successful, so more substrate Enzymes investigation coursework converted into product.
The kinetics of enzyme reactions differ somewhat from those of simple inorganic reactions. However, there are some factors that I must take into consideration. So therefore in order to overcome this error, I will be using a thermostatically controlled water bath. The temperature must be kept Enzymes investigation coursework otherwise the activity of the enzymes will fluctuate and so this will effect the volume of oxygen produced.
This means that more enzymes will be able to work at the same time to break down the hydrogen peroxide, and so reaction time will decrease as the enzyme concentration increases.
Many other enzymes catalyse other types of reactions. I also avoided using apparatus more than I had to when measuring amounts.
Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, Enzymes investigation coursework it was hard to read the correct values every time.
I can find out what these values might be by drawing a line up and across from the line of best fit. This is shown in the table Enzymes investigation coursework all the results I have gained Fig.
This would have affected the number of molecules of hydrogen peroxide present, which in turn would have affected the number of collisions between enzyme and substrate molecules. Groups of three could work to collect results for 5 different concentrations and rotate the roles of apparatus manipulator, result reader and scribe.
Evaluation Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total. Minute quantities of an enzyme can accomplish at low temperatures what would require violent reagents and high temperatures by ordinary chemical means.
This would show that the reaction is a first order reaction. Overall, there is a pattern showing a constant trend in that as the concentration decreases the rate of reaction decreases too, and that the overall volume of gas evolved also decreases.
Similarly, if the stopwatch is started after the reaction has started to take place then, the results will be unreliable because they will correspond with the wrong time frame.
Then the rate of reaction is calculated. Each enzyme is selectively specific for the substance in which it causes a reaction and is most effective at a temperature peculiar to it. This should tell me whether the reaction is indeed a first-order reaction, or if a curve of best fit is required.
This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed. Apparatus Limitations Firstly, there were limitations on the apparatus that I used. Hence the percentage error was: This is shown when oxygen stops being produced and the same results are recorded five times.
Keep in brown bottles because hydrogen peroxide degrades faster in the light. As result of this I will be using a burette to collect the volume of oxygen as a burette will provide more accurate results.
Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously. The line of best fit also suggests values of concentrations which I have not investigated. Use a cm3 measuring cylinder for concentrations of hydrogen peroxide over 20 vol.
As I have mentioned earlier, this decreases in steps of 3. I will keep the volume of hydrogen peroxide at 1cm3.ENZYME. INVESTIGATION. AQA Biology Dual Award. Skills Tested: Observation /8.
Analysis /8. Evaluation /6. The Skinners’ School. 1. Introduction. Enzymes were discovered by a German chemist called Eduard Buchner near the end of. Apr 30, · Here is an example of A-level biology coursework on the effect of substrate concentration (hydrogen peroxide) on the rate of activity of the enzyme catalase.
I have identified problems that may occur in my main investigation, such as timing, measuring and keeping variables that I am not investigating constant.
The curve will Reviews: 5. Factors affecting enzyme activity.
Enzymes are sophisticated catalysts for biological processes. These practicals (and the practicals at intermediate level) give you opportunities to explore how enzyme activity changes in different conditions.
Below is an essay on "Investigating Enzymes Coursework" from Anti Essays, your source for research papers, essays, and term paper examples.
Investigating Enzymes Aim: To investigate how temperature affects the rate of reaction in enzymes.
Read Enzymes Coursework free essay and over 88, other research documents. Enzymes Coursework. ENZYME INVESTIGATION Planning Introduction: An Enzyme is any one of many specialised organic substances, composed of polymers of amino acids, /5(1).
Biology Coursework Enzymes. Extracts from this document Introduction. The enzyme amylase that I will be using throughout my investigation to find out how starch concentration affects an enzyme controlled reaction is found in saliva in the mouth and also in the small intestine.
Inside the body the enzyme amylase is used to break down 4/5.Download